Enzymes are useful biocatalyst in many industrial processes. Recent innovations in the area of protein engineering has created the opportunity for production of new enzymes tailor-made for entirely new area of applications. Use of enzymes as industrial biocatalyst offers a range of advantages over traditional and hazardous process with respect to its process efficiency, exceptional product selectivity, reduced time and cost, improved yield and high market profitability. Majority of the industrial-enzymes are produced using microorganism including bacteria, yeast and fungi and many of these enzymes have proven their utility in Pharmaceutical and analytical industry, Food industry, Baking industry, Dairy industry, Beverages industry, Feed industry, Polymer industry, Paper and Pulp industry, Leather industry, Textile industry, Organic synthesis industry, Cosmetics, Detergents and Waste treatment. The demand for industrial-scale up of microbial enzymes is growing rapidly as these enzymes are proven to have enormous potential in various industries to meet the demand of growing population and cope exhaustion of natural resources.